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A26487
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A10663
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| Short Description | 10 kD Standard | IgG Purity and Heterogeneity Kit |
| Long Description | 10 kDa marker used as a suitability reference for the (P/N A10663) IgG Purity/Heterogeneity Kit and (P/N 390953) SDS-MW Kit. | The IgG Purity and Heterogeneity Kit is designed for use on the PA 800 Plus and is used to assess the purity and heterogeneity of IgG molecules in both a reduced and non-reduced state. The methodology involves heat denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS. These products are separated using a replacable gel matrix within a capillary for separation. This kit will detect impurities as low as 0.1% and includes a standard, IgG control with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns. This kit provides reagent volumes for analysis of 100 samples and includes: • Separation Capillary, 67 cm x 50 µm ID Bare-Fused Silica, (2) • SDS Gel Separation Buffer, 140 mL • SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL • IgG Control Standard, 1 mg/mL in SDS sample buffer , 1 mL • Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL • Acidic Wash Solution, 0.1 N HCl, 100 mL • Basic Wash Solution, 0.1 N NaOH, 100 mL |
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