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B94499PTO
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477600
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| Short Description | Fast Glycan Labeling and Analysis Kit | Carbohydrate Labeling & Analysis Kit |
| Long Description | The Fast Glycan Labeling and Analysis kit is designed for use on the PA 800 Plus and is used to rapidly label glycoprotein released N-Glycans with APTS followed by the effective removal of the excess dye using a magnetic bead-based clean-up. The labeled glycans are separated by high-resolution capillary electrophoresis utilizing laser-induced flourescence (LIF) detection and the HR-NCHO separation gel. Glycan identification is determined based on their normalized electrophoretic mobility referenced against pre-determined GU values using the appropriate bracketing standard. This kit provides reagent volumes for analysis of 100 samples and includes: • M1, 22 mL • D1, 0.05 mL x 5 vials • D2, 250mM/50 µL dried x 5 vials • D3, 1.5 mL x 1 vial • D4, 1.5 mL x 2 vials • L5, 5 mg x 1 vial • L6, 5 mg x 5 vials • GU Ladder, 5 mg x 1 vial • IST (Internal Standard), 5 mg x 1 vial • BST (Bracketing Standard), 10 pmol x 1 vial • HR-NCHO Separation Gel, 56ml • Magnetic Separator, 1 unit • Pre-Assembled Cartridge (P/N A55625), 1 unit | The Carbohydrate Labeling and Analysis Kit is designed for use on the PA 800 Plus and contains the reagents, buffers, and capillaries required to label, separate, and quantify oligosaccharides and monosaccharides released from glycoproteins. After enzymatic or chemical release, sugars are labeled with a fluorophore (APTS) at the reducing termini by reductive amination. The stoichiometry of labeling is such that only one APTS molecule is attached to each molecule of oligosaccharide. These highly charged and fluorescent oligosaccharides are easily resolved in an electric field and detected by laser-induced fluorescence (LIF) detection. This kit provides reagent volumes for analysis of 100 samples and includes: • Carbohydrate Separation Buffer, 56 mL • N-CHO Coated Capillary, 2 units • Labeling Reagents, 1 vial • Labeling Dye (APTS), 4 x 5 mg • Labeling Dye Solvent, 1 mL • Glucose Ladder Standard, 50 mg • Quantitation/Mobility Marker (Maltose), 0.18 mg • APTS-M (monosaccharide-grade), 20 mg |
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